Coding
lacI ts
Part:BBa_J06501
Designed by: ytwang Group: iGEM2005 (2005-06-14)
LacI repressor (temperature-sensitive mut 265) (+LVA)
Mutated from BBa_C0012 Repressor, LacI (LVA+) to be temperature-sensitive. Mutation from Chao, et al., 2002: Gly265->Asp (GG(803)T->GAT)
Sequence and Features
Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1147
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
direction | Forward |
function | Rep |
ligands | allolactose, IPTG |
protein | LacI-mut2 |
swisspro | ~ P03023 |
tag | LVA |